Detection of Giardia lamblia by immunofluorescence

Giardia Lamblia.

Lectin binding by Giardia lamblia.

Surface carbohydrates of Giardia lamblia were examined using six plant lectins chosen because of their specificity for major carbohydrates moieties. The binding to axenically grown G. lamblia trophozoites was assessed in both a quantitative microagglutination assay and a fluorescence assay. Of the six lectins tested, wheat germ agglutinin (WGA) agglutinated the highest percentage (22.9 +/- 3.7%...

Detection of Giardia lamblia Cysts in Surface Waters of Rasht City, Iran

Introduction: Giardia lamblia is a protozoan parasite with universal distribution in human populations. This infections transfer to human via contaminated foods and waters with Giardia cysts. The Knowledge on the incidence of this agent in the potential infection sources can provide valuable information for control and the spread of this parasite to human communities. This study was aimed to is...

Detection of Giardia lamblia cysts in non-fixed long-term stored human feces by direct immunofluorescence assay.

Giardia lamblia cysts in fecal specimens are detected by conventional morphological methods. The direct immunofluorescence monoclonal antibody assay (DFA) is also applied to detect G. lamblia cysts in feces, but little is known about the usefulness of DFA in fecal specimens stored under various conditions. The aim of the present study was to evaluate the DFA for detection of G. lamblia cysts an...

Biology of Giardia lamblia.

Giardia lamblia is a common cause of diarrhea in humans and other mammals throughout the world. It can be distinguished from other Giardia species by light or electron microscopy. The two major genotypes of G. lamblia that infect humans are so different genetically and biologically that they may warrant separate species or subspecies designations. Trophozoites have nuclei and a well-developed c...